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KMID : 1005220160310010061
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Journal of Embryo Transfer
2016 Volume.31 No. 1 p.61 ~ p.64
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Molecular Sexing and Species Identification of the Processed Meat and Sausages of Horse, Cattle and Pig
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Kim Yoo-Kyung
Kang Yong-Jun
Kang Geun-Ho
Seong Pil-Nam
Kim Jin-Hyoung
Park Beom-Young
Cho Sang-Rae
Jeong Dong-Kee
Oh Hong-Shik
Cho In-Cheol
Han Sang-Hyun
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Abstract
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We developed a polymerase chain reaction (PCR)-based molecular method for sexing and identification using sexual dimorphism between the Zinc Finger-X and -Y (ZFX-ZFY) gene and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for mitochondrial DNA (mtDNA) cytochrome B (CYTB) gene in meat pieces and commercial sausages from animals of different origins. Sexual dimorphism based on the presence or absence of SINE-like sequence between ZFX and ZFY genes showed distinguishable band patterns between male and female DNA samples and were easily detected by PCR analyses. Male DNA had two PCR products appearing as distinct two bands (ZFX and ZFY), and female DNA had a single band (ZFX). Molecular identification was carried out using PCR-RFLP of CYTB gene, and showed clear species classification results. The results yielded identical information on the sexes and the species of the meat samples collected from providers without any records. The analyses for DNA isolated from commercial sausage showed that pig was the major source but several sausages originated from chicken and Atlantic cod. Applying this PCR-based molecular method was useful and yielded clear sex information and identified the species of various tissue samples originating from livestock.
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KEYWORD
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species identification , molecular sexing , horse , cattle , pig
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